21065 Pierce Background Eliminator Kit, for eliminating background from X-ray film. 21059 Restore Western Blot Stripping Buffer, 500mL. 37535 SuperBlock Blocking Buffer in TBS, 1L. Thermo Scientific 1-Step NBT/BCIP Substrate Solution. Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions. 37515 SuperBlock Blocking Buffer in PBS, 1L. Thermo Scientific Pierce ECL Western Blotting Substrate. The Bio-Rad Clarity ECL substrate extends the signal duration to 24 hours, permitting reimaging without loss of signal intensity. 32106 Pierce ECL Western Blotting Substrate, 500mL. Luminol has an emission half-life of less than a minute. Introduction The Thermo Scientific Pierce ECL Western Blotting Substrate is a highly sensitive nonradioactive, enhanced luminol-based. The type of enhancer used can also increase the signal duration. For example, the enhancers used in Bio-Rad Clarity Max ECL substrate maximizes detection sensitivity to enable detection to the low femtogram amounts of target. Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. Clarity and Clarity Max ECL Substrates are compatible with any HRP conjugate and ideal for all western blotting. There are a number of different enhancers available, some of which can increase the signal by as much as a 1,000-fold, making ECL more sensitive than other common detection systems such as conversion of substrates to colored precipitates. This enhancement of a luminol-based signal is commonly referred to as enhanced chemiluminescence (ECL). However, luminol emits light only weakly, so enhancers are added to the reaction to increase the signal. Chromogenic no special equipment needed for visualization produces dark blue bands. Sensitive detection limit similar to Pierce ECL Western Blotting Substrate. Fade-resistant protein bands stable after membrane drying. This reaction emits light at 428 nm and this light signal can be captured on X-ray film or by a digital imager. Features of 1-Step Ultra TMB-Blotting Solution: Fast protein bands visible in less than one minute. The secondary antibody used in western blotting is conjugated to the horseradish peroxidase (HRP) enzyme which reacts with the HRP substrate luminol. Compare and learn more about our western blot chemiluminescent substrates View all HRP substrates available Pierce ECL Western Blotting Substrate provides. View ECL Blotting Substrates ECL Substrates for High-Sensitivity Western Blot DetectionĬhemiluminescent assays depend on the emission of light as a product of a chemical reaction and are commonly used for the detection of proteins on western blots. ![]() Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.Clarity and Clarity Max are compatible with any horseradish peroxidase (HRP) conjugate and ideal for all western blots with both digital and film-based imaging systems. Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Pierce Protein Methods Chemiluminescent detection for western blotting is popular because it offers several advantages over other detection methods. For chemiluminescent detection of AP, acridan- and 1,2-dioxetane-based substrates are available. Thermo Scientific Pierce ECL Western Blotting Substrate. Specifically, luminol- and acridan-based reagents are chemiluminescent HRP substrates. The choice of substrate for chemiluminescent western blotting is determined by the reporter enzyme that is selected. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Chemiluminescent western blot detection methods. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. ![]() Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
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